The Pan Probe Biotech, Inc., LiveSure^TM Methadone Screen is an in vitro diagnostic (IVD), qualitative, lateral flow, immunochromatographic urinary assay for the rapid detection of methadone, its analogs and its metabolites at the Substance Abuse and Mental Health Services Administration (SAMHSA) cut-off level of 300 ng/ml.  Methadone is a synthetic narcotic/analgesic drug substitute, usually taken orally or by intravenous or intramuscular injections.  Since dosing with methadone usually causes little euphoria but does appear to often result in physiologic dependency and tolerance, the drug has been widely prescribed for use in blocking opioid drug abuse.  Methadone is most often used in maintenance programs as a substitute for heroin or other abused opioids, permitting patients to be functionally successful at work and in life while remaining in a drug rehabilitation program. Overdose with methadone can result in drowsiness and confusion, especially when potentiated by ethyl alcohol, or other CNS depressant drugs.  The LiveSure^TM Methadone Screen Test is a competitive immunoassay in which a chemically modified and bound methadone conjugate competes with urinary methadone for limited, specific methadone antibody binding sites.  Each test device contains a sample reaction unit, a pink-colored, antibody-colloidal gold conjugate unit pre-labeled with specific antibody, and a chromatographic membrane pre-coated with drug conjugate in the device test region.